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	Provedor de dados Anais da ABC (AABC) (2) OAK (1) Autor ALMEIDA,ADÉLIA E. (1) Asada, Masahito (1) BRANDÃO,ADEILTON (1) CICARELLI,REGINA M.B. (1) Inoue, Noboru (1) Kawazu, Shin-ichiro (1) MAGALHÃES,NATHÁLIA O. (1) MARTINS,GILMARCIO Z. (1) MOREIRA,RAQUEL R.D. (1) PIETRO,ROSEMEIRE C.L.R. (1) SAMUDIO,FRANKLYN (1) SILVA,FLÁVIA A. J. (1) Suganuma, Keisuke (1) Yamagishi, Junya (1) Yamasaki, Shino (1) Yokoyama, Naoaki (1) Mais... Palavra-chave Epimastigote (3) Antitrypanosomal activity (1) Calmodulin (1) Hemoglobin receptor (1) Minimum free energy (1) RNA secondary structure (1) Solamargine (1) Solanum lycocarpum (1) Solanum palinacanthum (1) Trans-splicing (1) Trypanosoma cruzi (1) Trypanosome congolense (1) Untranslated region (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2016 (1) 2013 (1) País Brazil (2) Japan (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Characterization of an epimastigote-stage-specific hemoglobin receptor of Trypanosoma congolense OAK Yamasaki, Shino; Suganuma, Keisuke; Yamagishi, Junya; Asada, Masahito; Yokoyama, Naoaki; Kawazu, Shin-ichiro; Inoue, Noboru. Background: Since Trypanosorna spp. lack a complete heme synthesis pathway, the parasites are totally dependent on their host for heme throughout all of the stages of their life -cycle. We herein report the identification and characterization of a T. congolense epimastigote form (EMF)-specific hemoglobin (Hb) receptor. The gene was initially reported to encode a T. congolense haptoglobin (Hp)-Hb complex receptor (TcHpHbR) based on its similarity to a gene encoding a T brucei Hp-Hb complex receptor (TbHpHbR). Methods: Trypanosorna congolense IL3000 was used in this study. A TcHpHbR gene was PCR amplified from the parasite genome. The recombinant protein was used as an immunogen to raise antibodies for immunofluorescence assay and immunoblotting. Hemoglobin... Palavras-chave: Trypanosome congolense; Epimastigote; Hemoglobin receptor. Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4441 In vitro trypanocidal activity of solamargine and extracts from Solanum palinacanthum and Solanum lycocarpum of brazilian cerrado Anais da ABC (AABC) MOREIRA,RAQUEL R.D.; MARTINS,GILMARCIO Z.; MAGALHÃES,NATHÁLIA O.; ALMEIDA,ADÉLIA E.; PIETRO,ROSEMEIRE C.L.R.; SILVA,FLÁVIA A. J.; CICARELLI,REGINA M.B.. The present investigation was to evaluate the potential trypanocidal activity of crude ethanolic extract of the fruits of Solanum palinacanthum, Solanum lycocarpum and the glycoalcaloid, solamargine. S. palinacanthum and S. lycocarpum fruit powders were submitted to exhaustively extraction with 96% ethanol and solamargine were isolated from the extract of S. palinacanthum. Both extracts and solamargine were analysed for trypanocidal activity by using MTT colorimetric assay. Extracts of S. palinacanthum showed to be more active (IC50 = 175.9 µg.ml–1) than S. lycocarpum (IC50 = 194.7 µg.ml–1). Solamargine presented a strong activity (IC50 = 15.3 µg.ml–1), which can explain the better activity of the both extracts. Benznidazol (IC50 = 9.0 µg.ml–1) is the only... Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum palinacanthum; Solanum lycocarpum; Solamargine; Epimastigote; Antitrypanosomal activity; Trypanosoma cruzi. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300903 Minimum free energy predicted base pairing in the 39 nt spliced leader and 5’ UTR of calmodulin mRNA from Trypanosoma cruzi: influence of the multiple trans-splicing sites Anais da ABC (AABC) SAMUDIO,FRANKLYN; BRANDÃO,ADEILTON. ABSTRACT We analyzed the compositional changes and the stable base pairs in the predicted secondary structure of the 5’ UTR calmodulin mRNA in T. cruzi. The three copies of calmodulin in T. cruzi genome display variable position of the trans splicing sites and give rise to several mRNA that differs slightly on 5’ UTR composition in the epimastigote stage. We show that the pattern of high probability base pairs in the minimum free energy predicted secondary structures of the calmodulin 5’ UTR remains unchanged despite the nucleotide composition variation. However, the 39 nt spliced leader (mini-exon, the 5’ exon sequence transferred to trypanosome mRNAs by the mechanism of trans splicing) shows a variable pattern of high and low probability base pairing as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trans-splicing; Untranslated region; Calmodulin; Epimastigote; RNA secondary structure; Minimum free energy. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502311 Registros recuperados: 3 Primeira ... 1 ... Última

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